Glyco@Seminar: Juan C. Alonso

on the June 11, 2018

June 11th, 2:00pm
Juan C. Alonso from Centro Nacional de Biotecnologia (CSIC), Madrid, will give a talk about " Toxin-antitoxin system: impact on the bacterial peptidoglycan biosynthesis ".

I will present our recent study of on the characterization of the epsilon/zeta toxin–antitoxin (TA) module. In response to stress the antitoxin is degraded and the free toxin reversible induces cell dormancy. The epsilon/zeta TA module is ubiquitous in the genomes of many pathogenic bacteria. The TA complex binds the peptidoglycan precursor uridine diphosphate-N-acetylglucosamine (UNAG), and toxin zeta binds ATP and UNAG. Toxin zeta hydrolyses ATP and phosphorylates UNAG, and that these activities are counteracted by binding of epsilon antitoxin. Phosphorylated UNAG is unreactive to the MurAA/MurAB enzyme, thus the initial step in bacterial peptidoglycan biosynthesis should be blocked. I will discuss the apparent contradiction of zeta toxin inducing reversible dormancy and poisoning peptidoglycan biosynthesis.


Juan C. Alonso is a Research Professor at the Centro Nacional de Biotecnología at the Consejo Superior de Investigaciones Científicas (CNB-CSIC) in Spain. His research focuses on the characterization of the mechanisms that contribute to genetic stability in Bacillus subtilis. He received his B.Sc. in Veterinary Medicine and in Bacteriology and PhDs in Animal Nutrition and Biochemistry in the groups of Professor Eduardo Zaccardi (Veterinary Science) and Oscar Grau (Exact Science) at the Universidad de La Plata, Argentina. As a postdoctoral researcher he studied the mechanism of plasmid replication in B. subtilis, and a Research Group Leader he studied the mechanism of plasmid segregation at the Max-Planck Institute for Molecular Genetics, in Berlin Germany. Juan was appointed as a Professor Fellow of the Max-Planck Society (C3 position) at the Max-Planck Institute for Molecular Genetics, in Berlin Germany to study the mechanisms of viral DNA packaging. In 1994, he moves to the CNB-CSIC initially as Associated Professor and then as Research Professor until present.

Juan C. Alonso's page
Poster G Seminar Juan Alonso

Published on June 8, 2018

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